Collagen content dating

By increasing the rate of flow, one can increase the effect of the vacuum.A glass filter is placed at the bottom of the funnel and dampened with distilled water (dist. Afterwards, the sample is placed on top of the filter paper and a number of litres of distilled water poured in and drawn through the sample by the vacuum effect.The aim is to reduce the p H levels of the sample to a neutral level by continual rinsing.During this treatment, regular litmus readings are taken to determine the extent of the acidity remaining.HCl acid washing is usually applied to samples destined for combustion.Because HCl reacts with carbonate to produce CO2, its use in pretreatment work is restricted to non-carbonate samples.After approximately one hour it is removed and placed into a buchner funnel.The buchner apparatus uses the pressure of flowing water to create a vacuum.

This apparatus continually recycles the solvents being used so that they do not have to be replaced.

Many samples from terrestrial environments, such as wood, charcoal and peat, will often contain small amounts of absorbed carbonates from percolating groundwater.

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